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Hypothetical Protein Annotation Exam 3

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Hypothetical Protein Annotation Exam 3

icarus July 9, 2024

The following analysis uses a gene expression dataset of vancomycin treated and untreated cultures from 2 resistant Staphylococcus aureus strains – C1 and T8 – at various times throughout a growth curve. The dataset was acquired from NCBI’s Gene Expression Omnibus (NCBI identifier: GSE26400). To save time, the relevant information from the series matrix and the platform files have been cleaned and provided in the attached text files. Use the files to perform the following:

  • Use the 2 GSE26400data.txt files as input to iDEP and collect the needed results
  • Use “Conditional Formatting” to identify hypothetical proteins from both single-gene and singular enrichment analyses
  • Use GSEA and Excel to identify hypothetical proteins

Then answer the following questions