Hypothetical Protein Annotation Exam 2
Acquire the protein sequence for 4CLK from PDB and perform 3 iterations of PSI-BLAST on it to answer some of the following questions. The acquire the sequences for the following proteins: XP_032771309.1, XP_027833666.1, KAB1260710.1, CAD7674611.1, KAH1166356.1, MXQ95824.1, KAI4579396.1, and KAF5916588.1. Use all 9 protein sequences for multiple sequence alignment, phylogenetic tree generation, physiochemical characterization, cellular location prediction, and secondary and tertiary structure predictions, then answer the remaining questions.